Our lab has developed tools to aid in the generation of guide RNA CRISPR libraries, initially with GuideScan 1.0 (Perez, Pritykin, Vidigal et al., Nature Biotechnology). Since then, we have developed GuideScan 2.0 (Schmidt & Zhang et al., Genome Biology) which is used by labs worldwide (please see guidescan.com). These tools and others have led us to perform focused and whole-genome CRISPR and shRNA screens.
Leveraging our expertise in genome engineering in both mice and cell lines, we have taken efforts to understand several fundamental molecular mechanisms that drive tumorigenesis including a feedback circuit regulating MYC and the role of co-amplified genes on ecDNAs. We are currently pursuing both focused and whole-genome CRISPR knockout screens, shRNA-based screens, and base editor screens. Interested members are encouraged to pitch their own screens and areas of investigation.
