Selected Publications: Here
Complete Publications: Here
Full list of publications also available on: Google Scholar
2024
51. Nam, K.H. and Ordureau, A. (2024) How does the neuronal proteostasis network react to cellular cues? Biochem. Soc. Trans. 2024 Mar 15:BST20230316.
50. DaRosa, P.A., Penchev, I., Gumbin, S.C., Scavone, F., Wąchalska, M., Paulo, J.A., Ordureau, A., Peter, J.J., Kulathu, Y., Harper, J.W., Becker, T., Beckmann, R., Kopito, R.R. (2024) UFM1 E3 ligase promotes recycling of 60S ribosomal subunits from the ER. Nature Online ahead of print.
49. Watts, M.E., Giadone, R.M., Ordureau, A., Holton, K.M., Harper, J.W., Rubin, L.L. (2024) Analyzing the ER stress response in ALS patient derived motor neurons identifies druggable neuroprotective targets. Front. Cell. Neurosci. 17, 1327361.
48. Paul, S., Sarraf, S.A., Nam, K.H., Zavar, L., DeFoor, N., Biswas, S.R., Fritsch, L.E., Yaron, T.M., Johnson, J.L., Huntsman, E.M., Cantley, L.C., Ordureau, A., Pickrell, A.M. (2024) NAK-associated protein 1/NAP1 activates TBK1 to ensure accurate mitosis and cytokinesis. J Cell Biol. 223(2):e202303082.
47. Morrone Parfitt, G., Coccia, E., Goldman, C., Whitney, K., Reyes, R., Sarrafha, L., Nam, K.H., Sohail, S., Jones, D.R., Crary, J.F., Ordureau, A., Blanchard, J., Ahfeldt, T. (2024) Disruption of lysosomal proteolysis in astrocytes facilitates midbrain organoid proteostasis failure in an early-onset Parkinson’s disease model. Nat Commun. 15(1):447.
2023
46. Park, H.M., Le, L., Nguyen, T.T., Nam, K.H., Ordureau, A., Lee, J.E., Nguyen, T.V. (2023) The CRL3gigaxonin ubiquitin ligase-USP15 pathway governs the destruction of neurofilament proteins. Proc Natl Acad Sci USA. 120(45):e2306395120.
45. Yang, N., Wang, Y., Dai, P., Li, T., Zierhut, C., Tan, A., Zhang, T., Xiang, J.Z., Ordureau, A., Funabiki, H., Chen, Z., Deng, L. (2023) Vaccinia E5 is a major inhibitor of the DNA sensor cGAS. Nat Commun. 14(1):2898.
44. Jordan, V.N., Ordureau, A., An, H. (2023) Identifying E3 Ligase Substrates With Quantitative Degradation Proteomics. Chembiochem. e202300108.
43. Fiesel, F.C., Fričová, D., Hayes, C.S., Coban, M.A., Hudec, R., Bredenberg, J.M., Broadway, B.J., Markham, B.N., Yan, T., Boneski, P.K., Fiorino, G., Watzlawik, J.O., Hou, X., McCarty, A.M., Lewis-Tuffin, L.J., Zhong, J., Madden, B.J., Ordureau, A., An, A., Puschmann, A., Wszolek, Z.K., Ross, O.A., Harper, J.W., Caulfield, T.R., Springer, W. (2023) Substitution of PINK1 Gly411 modulates substrate receptivity and turnover. Autophagy. (6):1711-1732.
2022
42. Nam, K.H., Ordureau, A. (2022) Quantitative proteome remodeling characterization of two human reference pluripotent stem cell lines during neurogenesis and cardiomyogenesis. Proteomics. 2022 Oct;22(19-20):e2100246.
41. Goldsmith, J., Ordureau, A., Harper, J.W., Holzbaur, E.L.F. (2022) Brain-derived autophagosome profiling reveals the engulfment of nucleoid-enriched mitochondrial fragments by basal autophagy in neurons. Neuron. 110(6):967-976.e8.
40. Welsh, K.A., Bolhuis, D.L., Nederstigt, A.E., Boyer, J., Temple, B.R.S., Bonacci, T., Gu, L., Ordureau, A., Harper, J.W., Steimel, J.P., Zhang, Q., Emanuele, M.J., Harrison, J.S., Brown, N.G. (2022) Functional conservation and divergence of the helix-turn-helix motif of E2 ubiquitin-conjugating enzymes. EMBO J. 41(3):e108823.
2021
39. Antico, O.*, Ordureau, A.*, Stevens, M., Singh, F., Nirujogi, R.S., Gierlinski, M., Barini, E., Rickwood, M.L., Prescott, A., Toth, R., Ganley, I.G., Harper, J.W., Muqit, M.M.K. (2021) Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1. Science Advances. 7(46):eabj0722.
38. Ordureau, A.†, Kraus, F., Zhang, J., An, H., Park, S., Ahfeldt, T., Paulo, J.A., Harper, J.W.†. (2021) Temporal proteomics during neurogenesis reveals large-scale proteome and organelle remodeling via selective autophagy. Molecular Cell. 81(24):5082-5098.e11.
37. Ordureau, A., Yu, Q., Bomgarden., R.D., Rogers, J.C., Harper, J.W., Gygi, S.P., Paulo, J.A. (2021) Super Heavy TMTpro Labeling Reagent: An Alternative and Higher-Charge-State-Amenable Stable-Isotope-Labeled TMTpro Variant. J Proteome Res. 7;20(5):3009-3013.
36. Najafov, A., Luu, H.S., Mookhtiar, A.K., Mifflin, L, Xia, H.G., Amin, P.P., Ordureau, A., Wang H, Yuan J. (2021) RIPK1 Promotes Energy Sensing by the mTORC1 Pathway. Molecular Cell. 81(2):370-385.
2020
35. McKenna, M.J.*, Sim, S.I.*, Ordureau, A., Wei, L., Harper, J.W., Shao. S., Park E. (2020) The endoplasmic reticulum P5A-ATPase is a transmembrane helix dislocase. Science 369(6511).
34. Sinha, N.K.*, Ordureau, A.*, Best, K.M.*, Saba, J.A., Zinshteyn, B., Sundaramoorthy, 34. E., Fulzele, A., Garshott, D.M., Denk, T., Thoms, M., Paulo, J.A., Harper, J.W., Bennett, E.J., Beckmann, R., Green, R. (2020) EDF1 coordinates cellular responses to ribosome collisions. eLife 9, e58828.
33. An, H.*, Ordureau, A.*, Korner, M., Paulo, J.A., Harper, J.W. (2020) Systematic Quantitative Analysis of Ribosome Inventory During Nutrient Stress. Nature 583(7815), 303-309.
32. Martinez-Chacin, R.C., Bodrug, T., Bolhuis, D.L., Kedziora, K.M., Bonacci, T., Ordureau, A., Gibbs, M.E., Weissmann, F., Qiao, R., Grant, G.D., Cook, J.G., Peters, J-M., Harper, J.W., Emanuele, M.J., Brown, N.G. (2020) Ubiquitin chain elongating E2 UBE2S activates the RING E3 APC/C for substrate priming with UBE2C. Nature Structural & Molecular Biology 27(6):550-560.
31. Ordureau, A., Paulo, J.A., Zhang, J., An, H., Swatek, K.N., Cannon, J.R., Wan, Q., Komander, D., Harper J.W. (2020) Global landscape and dynamics of Parkin and USP30-dependent ubiquitylomes in iNeurons during mitophagic signaling. Molecular Cell. 77(5):1124-1142.
30. Ahfeldt, T., Ordureau, A., Bell, C., Sarrafha, L., Sun, C., Piccinotti, S., Grass, T., Parfitt, G.M., Paulo, J.A., Yanagawa, F., Uozumi, T., Kiyota, Y., Harper, J.W., Rubin, L.L. (2020) Pathogenic pathways in early onset autosomal recessive Parkinson’s disease discovered using isogenic human dopaminergic neurons. Stem Cell Reports. 14 (1), 75-90.
2019
29. Jacoupy, M., Hamon-Keromen, E., Ordureau, A., Erpapazoglou, Z., Coge, F., Corvol, J-C., Nosjean, O., Mannoury La Cour, C., Millan, M. J., Boutin, J. A., Harper, J. W., Brice, A., Guedin, D., Gautier, C. A., Corti, O. (2019) The PINK1 kinase-driven ubiquitin ligase Parkin promotes mitochondrial protein import through the presequence pathway in living cells. Scientific Reports. 9(1):11829.
28. Gottlieb, C.D.*, Thompson, A.C.S.*, Ordureau, A., Harper, J.W., Kopito, R.R. (2019) Acute unfolding of a single protein immediately stimulates recruitment of ubiquitin protein ligase E3C (UBE3C) to 26S proteasomes. J Biol Chem. 294 (45), 16511-16524.
27. Najafov, A., Mookhtiar, A.K., Luu, H.S., Ordureau, A., Pan, H., Amin, P.P., Li, Y., Lu, Q., Yuan, J. (2019) TAM Kinases Promote Necroptosis by Regulating Oligomerization of MLKL. Molecular Cell. 75(3):457-468.
26. An, H., Ordureau, A., Paulo, J.A., Shoemaker, C.J., Denic, V., Harper, J.W. (2019) TEX264 is an ER-resident ATG8-interacting protein critical for endoplasmic reticulum remodeling during nutrient stress. Molecular Cell. 74(5):891-908.
2018
25. Heo, J.M., Ordureau, A., Swarup, S., Paulo, J.A., Shen, K., Sabatini, D.M., Harper, J.W. (2018) RAB7A phosphorylation by TBK1 promotes mitophagy via the PINK-PARKIN pathway. Science Advances. Nov 21;4(11).
24. Ordureau, A., Paulo, J.A., Zhang, W., Ahfeldt, T., Zhang, J., Cohn, E.F., Hou, Z., Heo, 24. J.M., Rubin, L.L., Sidhu, S.S., Gygi, S.P., Harper, J.W. (2018) Dynamics of PARKIN-Dependent Mitochondrial Ubiquitylation in Induced Neurons and Model Systems Revealed by Digital Snapshot Proteomics. Molecular Cell. 70(2) 211-227.
23. Harper, J.W., Ordureau, A., Heo, J.M. (2018) Building and decoding ubiquitin chains for mitophagy. Nat Rev Mol Cell Biol. 19(2):93-108.
2017
22. Mohideen, F., Paulo, J.A., Ordureau, A., Gygi, S.P., Harper, J.W. (2017) Quantitative Phospho-proteomic Analysis of TNFα/NFκB Signaling Reveals a Role for RIPK1 Phosphorylation in Suppressing Necrotic Cell Death. Mol Cell Proteomics. 16(7):1200-1216.
21. Wang, B., Jie, Z., Joo, D., Ordureau, A., Liu, P., Gan, W., Guo, J., Zhang, J., North, B.J., Dai, X., Cheng, X., Bian, X., Zhang, L., Harper, J.W., Sun, S.C., Wei, W. (2017) TRAF2 and OTUD7B govern a ubiquitin-dependent switch that regulates mTORC2 signalling. Nature, 545(7654):365-369.
20. Liu L, Michowski W, Inuzuka H, Shimizu K, Nihira NT, Chick JM, Li N, Geng Y, Meng AY, Ordureau A, Kołodziejczyk A, Ligon KL, Bronson RT, Polyak K, Harper JW, Gygi SP, Wei W, Sicinski P. (2017) G1 cyclins link proliferation, pluripotency and differentiation of embryonic stem cells. Nat Cell Biol. 19(3):177-188.
2016
19. Rose C.M.*, Isasa M.*, Ordureau A., Prado M.A., Beausoleil S.A., Jedrychowski M.P., Finley D.J., Harper J.W., Gygi S.P. (2016). Highly Multiplexed Quantitative Mass Spectrometry Analysis of Ubiquitylomes. Cell Systems 3(4):395-403.
18. Brown, N. G.*, VanderLinden, R.*, Watson, E. R.*, Weissmann, F., Ordureau, A., Wu, K.-P., Zhang, W., Yu, S., Mercredi, P.Y., Harrison, J.S., Davidson, I.F., Qiao, R., Lu, Y., Dube, P., Brunner, M.R., Grace, C.R., Miller, D.J., Haselbach, D., Jarvis, M.A., Yamaguchi, M., Yanishevski, D., Petzold, G., Sidhu, S.S., Kuhlman, B., Kirschner, M.W., Harper, J.W., Peters, J.M., Stark, H., Schulman, B.A. (2016). Dual RING E3 Architectures Regulate Multiubiquitination and Ubiquitin Chain Elongation by APC/C. Cell 165(6), 1440–1453.
17. Zhang, W.*, Wu, K.-P.*, Sartori, M.A., Kamadurai, H.B., Ordureau, A., Jiang, C., Mercredi, P.Y., Murchie, R., Hu, J., Persaud, A., Mukherjee, M., Li, N., Doye, A., Walker, J.R., Sheng, Y., Hao, Z., Li, Y., Brown, Y.K., Lemichez, E., Chen, J., Tong, Y., Harper, J.W., Moffat, J., Rotin, D., Schulman, B.A., and Sidhu, S.S (2016). System-Wide Modulation of HECT E3 Ligases with Selective Ubiquitin Variant Probes. Molecular Cell 62, 121–136.
2015
16. Heo, J.H., Ordureau, A., Paulo, J.A., Rinehart, J., and Harper, J.W. (2015) The PINK1-PARKIN Mitochondrial Ubiquitylation Pathway Drives a Program of OPTN/NDP52 Recruitment and TBK1 Activation to Promote Mitophagy. Molecular Cell 60, 7–20.
15. Huttlin, E.L., Ting, L., Bruckner, R.J., Gebreab, F., Gygi, M.P., Szpyt, J., Tam, S., Zarraga, G., Colby, G., Baltier, K., Dong, R., Guarani, V., Vaites, L.P., Ordureau, A., Rad, R., Erickson, B.K., Wuhr, M., Chick, J., Zhai, B., Kolippakkam, D., Mintseris, J., Obar, R.A., Harris, T., Artavanis-Tsakonas, S., Sowa, M.E., De Camilli, P., Paulo, J.A., Harper, J.W., and Gygi, S.P. (2015). The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell 162, 425-440.
14. Pickrell, A.M.*, Huang, C.H.*, Kennedy, S.R., Ordureau, A., Sideris, D.P., Hoekstra, J.G., Harper, J.W., and Youle, R.J. (2015). Endogenous Parkin Preserves Dopaminergic Substantia Nigral Neurons following Mitochondrial DNA Mutagenic Stress. Neuron 87, 371-381.
13. Ordureau, A., Munch, C., and Harper, J.W. (2015). Quantifying ubiquitin signaling. Molecular Cell 58, 660-676.
12. Ordureau, A., Heo, J.M., Duda, D.M., Paulo, J.A., Olszewski, J.L., Yanishevski, D., Rinehart, J., Schulman, B.A., and Harper, J.W. (2015). Defining roles of PARKIN and ubiquitin phosphorylation by PINK1 in mitochondrial quality control using a ubiquitin replacement strategy. Proc Natl Acad Sci USA 112, 6637-6642.
11. Cirulli, E.T., Lasseigne, B.N., Petrovski, S., Sapp, P.C., Dion, P.A., Leblond, C.S., Couthouis, J., Lu, Y.F., Wang, Q., Krueger, B.J., Ren, Z., Keebler, J., Han, Y., Levy, S.E., Boone, B.E., Wimbish, J.R., Waite, L.L., Jones, A.L., Carulli, J.P., Day-Williams, A.G., Staropoli, J.F., Xin, W.W., Chesi, A., Raphael, A.R., McKenna-Yasek, D., Cady, J., Vianney de Jong, J.M., Kenna, K.P., Smith, B.N., Topp, S., Miller, J., Gkazi, A., Consortium, F.S., Al-Chalabi, A., van den Berg, L.H., Veldink, J., Silani, V., Ticozzi, N., Shaw, C.E., Baloh, R.H., Appel, S., Simpson, E., Lagier-Tourenne, C., Pulst, S.M., Gibson, S., Trojanowski, J.Q., Elman, L., McCluskey, L., Grossman, M., Shneider, N.A., Chung, W.K., Ravits, J.M., Glass, J.D., Sims, K.B., Van Deerlin, V.M., Maniatis, T., Hayes, S.D., Ordureau, A., Swarup, S., Landers, J., Baas, F., Allen, A.S., Bedlack, R.S., Harper, J.W., Rouleau, G.A., Brown, R., Harms, M.B., Cooper, G.M., Harris, T., Myers, R.M., and Goldstein, D.B. (2015). Exome sequencing in amyotrophic lateral sclerosis identifies risk genes and pathways. Science 347, 1436-1441.
2014
10. Li, N., Fassl, A., Chick, J., Inuzuka, H., Li, X., Mansour, M.R., Liu, L., Wang, H., King, B., Shaik, S., Gutierrez, A., Ordureau, A., Otto, T., Kreslavsky, T., Baitsch, L., Bury, L., Meyer, C.A., Ke, N., Mulry, K.A., Kluk, M.J., Roy, M., Kim, S., Zhang, X., Geng, Y., Zagozdzon, A., Jenkinson, S., Gale, R.E., Linch, D.C., Zhao, J.J., Mullighan, C.G., Harper, J.W., Aster, J.C., Aifantis, I., von Boehmer, H., Gygi, S.P., Wei, W., Look, A.T., and Sicinski, P. (2014). Cyclin C is a haploinsufficient tumour suppressor. Nat Cell Biol 16, 1080-1091.
9. Ordureau, A., Sarraf, S.A., Duda, D.M., Heo, J.M., Jedrychowski, M.P., Sviderskiy, V.O., Olszewski, J.L., Koerber, J.T., Xie, T., Beausoleil, S.A., Wells, J.A., Gygi, S.P., Schulman, B.A., and Harper, J.W. (2014). Quantitative proteomics reveal a feedforward mechanism for mitochondrial PARKIN translocation and ubiquitin chain synthesis. Molecular Cell 56, 360-375.
8. Ordureau, A., and Harper, J.W. (2014). Cell biology: balancing act. Nature 510, 347-348.
2013
7. Emmerich, C.H., Ordureau, A., Strickson, S., Arthur, J.S., Pedrioli, P.G., Komander, D., and Cohen, P. (2013). Activation of the canonical IKK complex by K63/M1-linked hybrid ubiquitin chains. Proc Natl Acad Sci USA 110, 15247-15252.
6. Ordureau, A., Enesa, K., Nanda, S., Le Francois, B., Peggie, M., Prescott, A., Albert, P.R., and Cohen, P. (2013). DEAF1 is a Pellino1-interacting protein required for interferon production by Sendai virus and double-stranded RNA. J Biol Chem 288, 24569-24580.
2012
5. Enesa, K., Ordureau, A., Smith, H., Barford, D., Cheung, P.C., Patterson-Kane, J., Arthur, J.S., and Cohen, P. (2012). Pellino1 is required for interferon production by viral double-stranded RNA. J Biol Chem 287, 34825-34835.
2011
4. Gleason, C.E., Ordureau, A., Gourlay, R., Arthur, J.S., and Cohen, P. (2011). Polyubiquitin binding to optineurin is required for optimal activation of TANK-binding kinase 1 and production of interferon beta. J Biol Chem 286, 35663-35674.
3. Nanda, S.K., Venigalla, R.K., Ordureau, A., Patterson-Kane, J.C., Powell, D.W., Toth, R., Arthur, J.S., and Cohen, P. (2011). Polyubiquitin binding to ABIN1 is required to prevent autoimmunity. J Exp Med 208, 1215-1228.
2010
2. Nichols, R.J., Dzamko, N., Morrice, N.A., Campbell, D.G., Deak, M., Ordureau, A., Macartney, T., Tong, Y., Shen, J., Prescott, A.R., and Alessi, D.R. (2010). 14-3-3 binding to LRRK2 is disrupted by multiple Parkinson’s disease-associated mutations and regulates cytoplasmic localization. Biochem J. 430, 393-404.
2008
1. Ordureau, A.*, Smith, H.*, Windheim, M., Peggie, M., Carrick, E., Morrice, N., and Cohen, P. (2008). The IRAK-catalysed activation of the E3 ligase function of Pellino isoforms induces the Lys63-linked polyubiquitination of IRAK1. Biochem J. 409, 43-52.