Research ArticlesBio/Publications at ORCID: 0000-0002-9163-6092Kochańczyk, T., Hann, Z.S., Lux, M.C., Delos Reyes, A.M.V., Ji, C., Tan, D.S., and Lima, C.D. Structural basis for transthiolation intermediates in the ubiquitin pathway. Nature (2024) doi: 10.1038/s41586-024-07828-9Puno, M.R., Lima, C.D. Structural basis for RNA surveillance by the human nuclear exosome targeting (NEXT) complex. Cell 185, 2132-2147 (2022) doi: 10.1016/j.cell.2022.04.016Das, M., Zattas, D., Zinder, J.C., Wasmuth, E.V., Henri, J., Lima, C.D. Substrate discrimination and quality control require each catalytic activity of TRAMP and the nuclear RNA exosome. Proc Natl Acad Sci U S A. 118:e2024846118. (2021) [PMID: 33782132]Hann, Z.S., Ji, C., Olsen, S.K., Lu, X., Lux, M.C., Tan, D.S., Lima, C.D.Structural basis for adenylation and thioester bond formation in the ubiquitin E1. Proc Natl Acad Sci U S A. 116, 15475-15484. (2019) [PMID: 31235585]Weick, E.M., Puno, M.R., Januszyk, K., Zinder, J.C., DiMattia, M.A., Lima, C.D. Helicase-Dependent RNA Decay Illuminated by a Cryo-EM Structure of a Human Nuclear RNA Exosome-MTR4 Complex. Cell 173, 1663-1677 (2018) [PMID: 29906447]Wasmuth, E.V., Zinder, J.C., Zattas, D., Das, M., and Lima, C.D. Structure and reconstitution of yeast Mpp6-nuclear exosome complexes reveals that Mpp6 stimulates RNA decay and recruits the Mtr4 helicase. Elife Jul 25;6. pii: e29062. doi: 10.7554/eLife.29062 (2017) [PMID: 28742025]Streich FC Jr, Lima CD. Capturing a substrate in an activated RING E3/E2-SUMO complex. Nature 536, 304-308 (2016) [PMID: 27509863]Zinder, J.C., Wasmuth, E.V., and Lima, C.D. Nuclear RNA exosome at 3.1 A reveals substrate specificities, RNA paths, and allosteric inhibition of Rrp44. Mol Cell 64, 734-735. (2016) [PMID: 27818140]Cappadocia, L., Pichler, A., and Lima, C.D. Structural basis for catalytic activation by the human ZNF451 SUMO E3 ligase. Nat Struct Mol Biol 22, 968-975. (2015) [PMID: 26524494]Bekes, M., van der Heden van Noort, G.J., Ekkebus, R., Ovaa, H., Huang, T.T., and Lima, C.D. Recognition of Lys48-linked di-ubiquitin and deubiquitinating activities of the SARS coronavirus papain-like protease. Mol Cell 62, 572-585. (2016) [PMID: 27203180]Wasmuth EV, Januszyk K, Lima CD. Structure of an Rrp6-RNA exosome complex bound to poly(A) RNA. Nature 511, 435-439 (2014) [PMID: 25043052]Armstrong AA, Mohideen F, Lima CD. Recognition of SUMO-modified PCNA requires tandem receptor motifs in Srs2. Nature 483, 59-63 (2012) [PMID: 22382979]Review ArticlesWeick, E.M. and Lima, C.D. RNA helicases are hubs that orchestrate exosome-dependent 3’-5’ decay. Curr Opin Struct Biol 67, 86-94 (2020) [PMID: 33147539]Puno, M.R., Weick, E.M., Das, M., Lima, C.D. Snapshot: The RNA exosome. Cell 179, 282-282 (2019) [PMID: 31539497]Cappadocia, L., Lima, C.D. Ubiquitin-like Protein Conjugation: Structures, Chemistry and Mechanism. Chem Rev. 118, 889-918 (2018) [PMID: 28234446]