James A. Fagin: Overview
We have focused in particular on the role of MAP kinase effectors in thyroid tumor initiation in sporadic and radiation-induced thyroid cancers, because these tumors are associated with a high prevalence of non-overlapping activating mutations of at least six genes encoding effectors in the pathway: the tyrosine kinase receptors RET and NTRK, the three RAS genes and BRAF. More recently, we have begun to explore the role of PI3K activation as a transitional event required for tumor progression.
Our present effort is in the following areas:
- Development and refinement of animal models of thyroid cancer to further probe the signaling pathways required for transformation. We have developed mouse models that recapitulate the genetic changes in thyroid cancer in a physiologically appropriate manner, by using conditional gene knock-in and deletion approaches, and are using them to determine the role of oncoprotein signal intensification in the development of oncogene addiction, and to establish the key pathways required for tumor viability.
- Identification of expression targets of oncogenic RET, RAS and BRAF that may account for critical phenotypic changes associated with cancers harboring the respective mutant genes.
- Exploring the role of innate immune cells in modulating thyroid cancer behavior in mouse models of the disease.
- Characterization of small molecule kinase inhibitors with potential therapeutic potential.