Our mission is the development of a “new” generation of optical imaging technologies to enable advances in two hitherto difficult-to-investigate areas: the real-time analysis of macromolecular interactions and motions at the nanometer scale in vivo and the three-dimensional architecture of complex molecular machines and of subcellular ultrastructure, in situ. We are also refining and applying ultrahigh-resolution spectroscopy techniques to dissect multistep complex biochemical processes using in vitro reconstituted single-molecule assays.
Alexandros Pertsinidis, PhD
Research FocusThe Pertsinidis laboratory uses single-molecule approaches to understand gene transcription and the function of complex macromolecular machines inside live cells.
EducationPhD, Brown University
- Jieru Li, Ankun Dong, Kamola Saydaminova, Hill Chang, Guanshi Wang, Hiroshi Ochiai, Takashi Yamamoto and Alexandros Pertsinidis. Single-Molecule Nanoscopy Elucidates RNA Polymerase II Transcription at Single Genes in Live Cells, Cell (2019).
- Guanshi Wang, Jesse Hauver, Zachary Thomas, Seth A. Darst, Alexandros Pertsinidis. Single-Molecule Real-Time 3D Imaging of the Transcription Cycle by Modulation Interferometry. Cell. Volume 167, Issue 7, p1839–1852.e21, 15 December 2016