Gerstner Sloan Kettering Core Course Topics

Gerstner Sloan Kettering Core Course Topics


Fall Semester

Section I – Genome Biology and Proteins
  • Chromosome structure
  • Cancer as a disease
  • Nature of mutations
  • Forward and reverse genetics
  • Linkage analysis
  • Protein structure, folding and modification
  • Protein structure-function analysis
  • Protein-protein interactions
  • Protein-nucleic acid interactions
  • Topoisomerases and the effects of DNA topology
  • Topoisomerase poisons and nucleoside metabolism effectors as chemotherapeutic agents
  • Drug discovery and development
  • Chromosome replication
  • DNA repair
  • Double-strand break repair and genome editing: Cas9 and other tools
  • Meiotic recombination
  • Biochemical mechanisms of homologous recombination
  • Detecting Chromosomal Structural Rearrangements 
Section II – Gene Expression
  • RNA modification
  • Transcription
  • Epigenetics
  • Translation
  • RNAi and miRNAs
  • Measurement of gene expression
  • Gene transfer vectors: experimental and medical applications
  • Computational and systems biology
  • Methods of experimental perturbation of gene expression and function in culture
  • Mouse model systems
  • Clinical manipulation of gene expression
  • Errors in protein folding and cancer
Section III – Cell Biology and Cell Cycle Control
  • Visualization of cells and cellular components
  • Voltage gated channels
  • Cellular membrane structure and function I
  • Cytoskeletal architecture
  • Chemotaxis and Cell Migration
  • Cellular membrane structure and function II
  • Endoplasmic reticulum and endocytic trafficking
  • Mitochondria and endoplasmic reticulum
  • The cell cycle (G1 decisions)
  • The cell cycle (S-phase, once and only once per cycle)
  • Chromosome segregation and cell division
  • The mitotic checkpoint
  • Centrosome
  • p53
  • The DNA damage response
  • Chemical biology and chemotherapy of mitotic poisons
  • Programmed cell death
  • Cell cycle checkpoints and chemotherapy
  • Phase I design principles
  • Radiation as a therapeutic agent

Spring Semester

Section IV – Development, Stem Cells and Morphogenesis
  • Introduction to developmental biology
  • Preimplantation mouse development, cell lineages, and manipulation of the mouse genome in ES cells; basic tools of mouse molecular genetics
  • Gastrulation and early morphogenesis in mammals
  • Neural Development (cell polarity and migration)
  • Gastrulation and early morphogenesis in Drosophila
  • Lineage and cell polarity in development
  • The Ras and MAP kinase pathways: normal functions in development
  • The Ras and MAP kinase pathways in disease
  • TGFβ signaling pathways in development
  • TGFβ signaling pathway in developmental disorders, inflammation and cancer
  • Hedgehog in development
  • Hedgehog and cancer
  • Wnt—the pathway and its normal functions in development (canonical and non-canonical pathways)
  • Wnt and cancer
  • Notch
  • Cell migration and invasion
  • Growth regulation: PI3K and Hippo pathways in development
  • Akt and mTor in cancer
  • Crosstalk between signaling pathways
  • Epigenetic regulation in development
  • Cell-to-cell adhesion and communication
  • Cell matrix adhesion and signaling
  • Stem Cells
  • Stem Cells and human development
Section V – Immunology
  • Overview: molecules, cells and tissues
  • Innate immunity
  • Cytokines and chemokines
  • NK cels and receptors
  • MHC and antigen presentation
  • Rearrangements of antigen receptor genes
  • B cell differentiation
  • Lymphocyte activation
  • T cell differentiation
  • Tolerance and autoimmunity
  • Tumor immunity I: lymphoid architecture and recirculation
  • Tumor immunity II
  • Tumor immunity III
  • Biostatistics
Section VI – Cancer Biology
  • Cancer Genomics
  • Principles of biomarkers
  • Learning from Extraordinary Responders
  • Pathology slide review
  • Cancer metabolism
  • DNA Methylation and Oncogenic Transformation
  • Acute Myeloid Leukemia
  • Functional Genomics
  • Lymphoid Malignancies
  • Glioblastoma Multiforme
  • Computational Biology Lab Exercise
  • Thyroid Cancer-Mouse Models, Kinase Inhibitors, Determinants of Response and Mechanisms of Intrinsic Resistance
  • Sarcomas
  • Leukemia Stem Cells
  • Breast cancer and Targeted Therapies
  • Prostate cancer-translocations
  • Drug resistance
  • Cancer susceptibility
  • Kidney cancer
  • Genotype-directed clinical trials