James Muller, PhD

Advanced Imaging Specialist - Manager of Light Microscope Cluster

James Muller, PhD

Advanced Imaging Specialist - Manager of Light Microscope Cluster

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James Muller

Office Phone

212-639-8215

Office Fax

646-422-2355

2021-Present: Advanced Imaging Specialist, Developmental Biology & Immunology Programs, Sloan Kettering Institute

Education & Training

2018-2020: Postdoctoral Fellow, Memorial Sloan Kettering Cancer Center

2012-2018: PhD, Vilcek Institute of Graduate Biomedical Sciences, NYU School of Medicine

2011-2012 National Institute of Allergy and Infectious Disease Postbaccalaureate IRTA Fellow

2007-2011 BS in Biology and Chemistry, Delaware State University

Research Interests

My research interests include the application of cutting-edge light-sheet microscopy to answer basic biological questions at the tissue scale, but with cellular level resolution. As well, I am interested in tissue clearing methods that facilitate imaging normal and diseased states in whole organs or developing embryos.

As a postdoctoral fellow with Frederic Geissman in SKI’s Immunology program, I helped discover the origin of osteoclasts the bone resorbing macrophages and contributed to the development a novel treatment for osteopetrosis.

As a graduate student with Mike Dustin at the Skirball Institute at NYU I worked on studying the dynamics of the immunological synapse formation using TIRF, Single Molecule, and Super resolution imaging, methods.

Microscopy Training

I am available for training and technical advice on instruments located in the Light Microscopy Instrument Clusters (RRL1024, RRL624, ZRC1538), more information can be found at https://my.ilabsolutions.com/service_center/5708/?tab=about

Publications

Wu P, Heins ZJ, Muller JT, Katsnelson L, de Bruijn I, Abeshouse AA, Schultz N, Fenyö D, Gao J. (2019) Integration and Analysis of CPTAC Proteomics Data in the Context of Cancer Genomics in the cBioPortal. Mol Cell Proteomics. (9):1893-1898. doi: 10.1074/mcp.TIR119.001673. Epub 2019 Jul 15. PMID: 31308250; PMCID: PMC6731080.

Jacome-Galarza CE, Percin GI, Muller JT, Mass E, Lazarov T, Eitler J, Rauner M, Yadav VK, Crozet L, Bohm M, Loyher PL, Karsenty G, Waskow C, Geissmann F. (2019). Developmental origin, functional maintenance and genetic rescue of osteoclasts. Nature. 568(7753):541-545. doi: 10.1038/s41586-019-1105-7. Epub 2019 Apr 10. PMID: 30971820; PMCID: PMC6910203.

Muller J, Baeyens A, Dustin ML. (2018). Tumor Necrosis Factor Receptor Superfamily in T Cell Priming and Effector Function. Adv Immunol.140:21-57. doi: 10.1016/bs.ai.2018.08.001. Epub 2018 Sep 17. PMID: 30366518.

McDonald-Hyman C, Muller JT, Loschi M, Thangavelu G, Saha A, Kumari S, Reichenbach DK, Smith MJ, Zhang G, Koehn BH, Lin J, Mitchell JS, Fife BT, Panoskaltsis-Mortari A, Feser CJ, Kirchmeier AK, Osborn MJ, Hippen KL, Kelekar A, Serody JS, Turka LA, Munn DH, Chi H, Neubert TA, Dustin ML, Blazar BR. (2018).The vimentin intermediate filament network restrains regulatory T cell suppression of graft-versus-host disease. J Clin Invest. 128(10):4604-4621. doi: 10.1172/JCI95713. Epub 2018 Aug 14. PMID: 30106752; PMCID: PMC6159973.

Cai H, Muller J, Depoil D, Mayya V, Sheetz MP, Dustin ML, Wind SJ. (2018). Full control of ligand positioning reveals spatial thresholds for T cell receptor triggering. Nat Nanotechnol. 13(7):610-617. doi: 10.1038/s41565-018-0113-3. Epub 2018 Apr 30. PMID: 29713075; PMCID: PMC6035778.

Mendoza A, Fang V, Chen C, Serasinghe M, Verma A, Muller J, Chaluvadi VS, Dustin ML, Hla T, Elemento O, Chipuk JE, Schwab SR. (2017). Lymphatic endothelial S1P promotes mitochondrial function and survival in naive T cells. Nature. 546(7656):158-161. doi: 10.1038/nature22352. Epub 2017 May 24. PMID: 28538737; PMCID: PMC5683179.

Chen HH, Händel N, Ngeow J, Muller J, Hühn M, Yang HT, Heindl M, Berbers RM, Hegazy AN, Kionke J, Yehia L, Sack U, Bläser F, Rensing-Ehl A, Reifenberger J, Keith J, Travis S, Merkenschlager A, Kiess W, Wittekind C, Walker L, Ehl S, Aretz S, Dustin ML, Eng C, Powrie F, Uhlig HH. (2017). Immune dysregulation in patients with PTEN hamartoma tumor syndrome: Analysis of FOXP3 regulatory T cells. J Allergy Clin Immunol. 139(2):607-620.e15. doi: 10.1016/j.jaci.2016.03.059. Epub 2016 Jun 18. PMID: 27477328; PMCID: PMC5292998.

Cai H, Depoil D, Muller J, Sheetz MP, Dustin ML, Wind SJ. (2017). Spatial Control of Biological Ligands on Surfaces Applied to T Cell Activation. Methods Mol Biol. 1584:307-331. doi: 10.1007/978-1-4939-6881-7_18. PMID: 28255709. 

Azoulay-Alfaguter I, Strazza M, Peled M, Novak HK, Muller J, Dustin ML, Mor A. (2017). The tyrosine phosphatase SHP-1 promotes T cell adhesion by activating the adaptor protein CrkII in the immunological synapse. Sci Signal. 10(491):eaal2880. doi: 10.1126/scisignal.aal2880. PMID: 28790195.

Dustin ML, Muller J. (2016). CELL SIGNALING. Liquidity in immune cell signaling. Science. 352(6285):516-7. doi: 10.1126/science.aaf8179. PMID: 27126023.

Crites TJ, Padhan K, Muller J, Krogsgaard M, Gudla PR, Lockett SJ, Varma R. (2014). TCR Microclusters pre-exist and contain molecules necessary for TCR signal transduction. J Immunol. 193(1):56-67. doi: 10.4049/jimmunol.1400315. Epub 2014 May 23. PMID: 24860189; PMCID: PMC4096552.