Generation and evaluation of CAR T cells engineered to treat multiple myeloma.
The van den Brink lab at Memorial Sloan Kettering Cancer Center is looking for a highly motivated post-doc to work on a project involving novel T cell immunotherapies for multiple myeloma. Our lab focuses on a variety of research topics related to cancer immunotherapy, including hematopoietic stem cell transplantation, graft-versus-host disease, effects of the microbiota on transplant outcomes, immune reconstitution/thymic rejuvenation strategies, and CAR T cell therapy for various malignancies.
The CAR T cell group of the van den Brink lab designs novel CAR T cells targeting hematologic malignancies with the goal of improving T cell activity while reducing toxicities. We study CAR T cell activity in syngeneic mouse models and are currently developing xenograft mouse models. This postdoc position will focus on developing next-generation CAR T cells targeting human myeloma antigens with the goal of translating these CAR T cells into clinical application.
- Human T cell culture and viral transduction
- in vitro cell proliferation, protein expression, cell viability, immunophenotyping, and cytoxicity assays
- CRISPR/Cas9 and gene transfer
- Generation and maintenance of tumor cell lines and virus producing-packaging lines in culture
- Xenograft models of human myeloma in mice
- Mouse handling, injection, and harvesting tissues
- Multi-color flow cytometry
- Molecular cloning of CAR constructs
The van den Brink lab is located in the Mortimer B. Zuckerman Research Center of the Sloan Kettering Institute of MSKCC. It is part of the Immunology Program. MSKCC combines laboratories with a focus on basic as well as cancer research. MSKCC is located in a vibrant hub of world-renowned biomedical research institutions in New York′s Upper East Side, adjacent to Rockefeller University and the Weill Cornell Medical College. The vast resources within MSKCC and these surrounding institutions will help to facilitate the success of the applicant′s projects and enable to establish collaborations with other laboratories.
Salary is competitive and commensurate with experience. MSKCC offers dedicated housing for postdoctoral fellows. This is a 2-year appointment with possibility of renewal for up to 5 years based upon satisfactory job performance, continuing availability of funds, and ongoing operational needs.
- PhD, MD/PhD, or MD completed in cancer immunology, immunotherapy, T cell biology, cell engineering, or related field
- Excellent verbal and written communication skills
- Solid publication record
- Experience will mammalian cell culture and aseptic techniques is essential
- Experience with retroviral/lentiviral human T cell transduction methods is preferred
- Experience with multi-color flow cytometry panels is preferred
- Experience with mouse handling and procedures is advantageous, but not required