Primary cilia are microtubule-based organelles that extend from the surface of most vertebrate cells (Fig. 1). Our genetic studies revealed that Hedgehog signaling in mammals depends on this organelle. Because aberrant Hedgehog signaling causes human tumors and because there is a large set of human inherited syndromes (the ciliopathies) that disrupt primary cilia, we study the mechanisms that link Hedgehog signaling to the primary cilium and that control cilia formation.
Changes in cilia structure in different ways have distinct effects on Hedgehog signaling. For example, KIF7 is an evolutionarily conserved component of the Hh signaling pathway that is required to relay signals from the membrane protein Smoothened (Smo) to the transcription factors of the Ci/Gli family, and mouse Kif7 mutants show a mild expansion of Hh-dependent cell types in the neural tube (Fig. 1A). Mouse Kif7 mutants also make long, abnormal cilia (Fig. 1B). We showed that purified KIF7 protein binds directly to the plus-ends of growing microtubules, where it controls microtubule dynamics. In vivo, KIF7 localizes to cilia tips, where it organizes a compartment where Gli activation can be correctly regulated by Hh ligand (He et al., 2014).
The lab is currently interested in defining the signals that control whether or not cells in the animal make cilia. For example, we identified Tau Tubulin Kinase 2 (TTBK2) as an essential regulator of the initiation of ciliogenesis (Goetz et al., 2012). To study the control of cilia formation, we generated double transgenic animals in which cilia are marked with a red fluorescent protein and the basal bodies that template cilia are marked with green fluorescent protein. These studies have led us to discover that ciliogenesis is regulated by lineage-specific mechanisms that are recapitulated in stem cells derived from specific embryonic lineages (Bangs et al., 2015).
We continue to identify and characterize new mutations that affect neural patterning and ciliogenesis.